biotinylated anti il 17a mab  (R&D Systems)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Regulatory T Cell Vaccination without Autoantigen Protects against Experimental Autoimmune Encephalomyelitis

doi: 10.4049/jimmunol.178.3.1791

Figure Lengend Snippet: Oral vaccination with Salmonella-CFAH elicits TGF-β-producing Treg cells and IL-4-, IL-10-, and IL-13-producing Teff cells in contrast to IL-17-producing, but no Th2-type cytokine-producing, Teff cells upon vaccination with Salmonella vector. Cell-sorted CD25+CD4+ and CD25−CD4+ T cells from mice orally immunized with H696 or H647 were evaluated for cytokine production following anti-CD3 and anti-CD28 costimulation. Treg cells had reduced IFN-γ production but elevated TGF-β when compared with Teff cells. IL-4, IL-10, and IL-13 segregated with the Teff cells induced by vaccination with H696. Teff cells from H647-vaccinated mice did not produce any Th2-type cytokines but rather produced elevated levels of IFN-γ. H647-induced Treg cells did produce TGF-β (although less than H696-vaccinated mice) and IL-17. Thus, protection conferred by Salmonella-CFA/I-induced Treg cells is because of reduced IFN-γ production and increased TGF-β, as well as in part supported by immune deviation by the Teff cells. *, p < 0.001 represents differences in cytokine production between CD25+CD4+ and CD25−CD4+ T cells, and †, p < 0.001 represents differences in cytokine production between H696- and H647-sorted cells.

Article Snippet: After blocking with PBS plus 1% BSA for 2 h at 37°C, washed wells were incubated with cell culture supernatants at 4°C for 24 h. After washing, 5.0 μ g/ml biotinylated chicken anti-human TGF- β 1 Ab (RD Systems) or 0.5 μ g/ml biotinylated rat anti-mouse IL-17 Ab (clone TC11-8H4; BD Pharmingen) were added for 2 h at 37°C.

Techniques: Plasmid Preparation, Produced